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Nanoparticles’ (NPs) properties characterization. After fabrication, NPs were characterized for their physicochemical and biological properties using dynamic light scattering. No significant changes in A) size and B) polydispersity index (PDI) were observed. However, a significant decrease in C) zeta potential for leukosomes (Leuko) compared to liposomes (Lipo) was noticed. D) Representative cryo‐TEM images of Lipo and Leuko verified that no morphological changes occurred following membrane protein integration to the NPs. Scale bars = 100 nm. E) Western blots for leukocyte membrane protein markers: CD11b, CD18, CD47, and <t>CD45</t> indicated their membrane integration in Leuko but absence in Lipo. Results are shown as mean ± SEM. Unpaired t‐test was used to determine statistical probabilities * p ≤ 0.05 among means considered statistically significant, n = 5.
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Phenotype characterization of partially hypomethylated MSCs. The expression of CD105, CD90, CD45, and CD34 was analyzed by flow cytometry for normally methylated cells ( A ), cells treated with 2.5 μM ( B ), or 5 μM ( C ) of 5-Aza-dC. No significant differences were observed in the expression of CD markers between DMSO-treated cells and cells treated with 5-Aza-dC ( D ). Bars represent the average (±SD) of three independent measurements, ns , not significant. Comparison between means was performed by one-way ANOVA test

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Article Title: Conservative Hypomethylation of Mesenchymal Stem Cells and Their Secretome Restored the Follicular Development in Cisplatin-Induced Premature Ovarian Failure Mice

doi: 10.1007/s43032-023-01389-4

Figure Lengend Snippet: Phenotype characterization of partially hypomethylated MSCs. The expression of CD105, CD90, CD45, and CD34 was analyzed by flow cytometry for normally methylated cells ( A ), cells treated with 2.5 μM ( B ), or 5 μM ( C ) of 5-Aza-dC. No significant differences were observed in the expression of CD markers between DMSO-treated cells and cells treated with 5-Aza-dC ( D ). Bars represent the average (±SD) of three independent measurements, ns , not significant. Comparison between means was performed by one-way ANOVA test

Article Snippet: Phycoerthrin (PE)-conjugated mouse monoclonal antibodies of CD90, and CD34 or FITC-conjugated monoclonal antibodies of CD105 and CD45 were from (Biotechne R&D System, MN, USA).

Techniques: Expressing, Flow Cytometry, Methylation, Comparison

Nanoparticles’ (NPs) properties characterization. After fabrication, NPs were characterized for their physicochemical and biological properties using dynamic light scattering. No significant changes in A) size and B) polydispersity index (PDI) were observed. However, a significant decrease in C) zeta potential for leukosomes (Leuko) compared to liposomes (Lipo) was noticed. D) Representative cryo‐TEM images of Lipo and Leuko verified that no morphological changes occurred following membrane protein integration to the NPs. Scale bars = 100 nm. E) Western blots for leukocyte membrane protein markers: CD11b, CD18, CD47, and CD45 indicated their membrane integration in Leuko but absence in Lipo. Results are shown as mean ± SEM. Unpaired t‐test was used to determine statistical probabilities * p ≤ 0.05 among means considered statistically significant, n = 5.

Journal: Advanced Functional Materials

Article Title: Biomimetic Nanoparticles as a Theranostic Tool for Traumatic Brain Injury

doi: 10.1002/adfm.202100722

Figure Lengend Snippet: Nanoparticles’ (NPs) properties characterization. After fabrication, NPs were characterized for their physicochemical and biological properties using dynamic light scattering. No significant changes in A) size and B) polydispersity index (PDI) were observed. However, a significant decrease in C) zeta potential for leukosomes (Leuko) compared to liposomes (Lipo) was noticed. D) Representative cryo‐TEM images of Lipo and Leuko verified that no morphological changes occurred following membrane protein integration to the NPs. Scale bars = 100 nm. E) Western blots for leukocyte membrane protein markers: CD11b, CD18, CD47, and CD45 indicated their membrane integration in Leuko but absence in Lipo. Results are shown as mean ± SEM. Unpaired t‐test was used to determine statistical probabilities * p ≤ 0.05 among means considered statistically significant, n = 5.

Article Snippet: Antibodies for western blot rat anti‐CD11b (MAB11241), goat anti‐CD18 (AF2618), rabbit anti‐CD45 (EPR20033), goat anti‐CD47 (ab108415), anti‐rabbit IgG‐HRP, anti‐goat IgG‐HRP, and anti‐rat IgG (Bio‐Techne Corporation, Minnesota, USA).

Techniques: Zeta Potential Analyzer, Liposomes, Membrane, Western Blot